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Future modeling efforts on CAM metabolism may provide more evidence to debate the subject. It can be extremely informative to combine genomic info, evolutionary modeling and genome-wide models to investigate possible points of evolutionary contingency. In addition, mathematically managed comparisons can be utilized to grasp the evolutionary limits of the assorted metabolic varieties (Alves and Savageau, 2000; Schwacke and Voit, 2004). Another area of alternative for CAM models is to help in the rational implementation of CAM metabolism in C3 crops. Recently, a set of thirteen CAM enzymes had been overexpressed independently in Arabidopsis (Lim et al., 2019).

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Models can video chat with other models and tip each other. The convergence of various FBA models to CAM flux distributions provides formal mathematical proof for low Ci as the principle driver for the evolution of CAM. We believe that using these models along with evolutionary models and genomic knowledge will shed more mild on CAM evolution and help rational engineering of CAM into C3 crops. Tay et al. used the Shameer et al. model to study the C3-to-CAM continuum. They simulated CAM idling and CAM cycling—both thought of as weak CAM—by impeding CO2 uptake in the course of the night time and through the whole cycle, respectively.

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There are two different pathways for malate decarboxylation, depending on the species. One starts with the release of CO2 by the conversion of malate to pyruvate by NAD-ME, adopted by the regeneration of PEP by PPDK (Kondo et al., 2000). In a second pathway, NAD-MDH converts malate to OAA, adopted by PEPCK, regenerating PEP, and releasing CO2 .